Proteinase K Cas 39450-01-6
  • Cas No.:39450-01-6
  • Molecular Formula:C29H27N2O12P
  • Molecular Weight:626.504641
  • Assay(%):≥95% (Native-PAGE)
  • Package:10g; 20g; 50g; 100g/bottle
  • ItemStandard
    Appearancewhite lyophilized powder
    Purity≥95% (Native-PAGE)
    Specific activity valueDry powder 40 U/mg protein, containing 50% lyoprotectant
    Dilution buffer20 mM Tris-Cl, 1mM CaCl2, pH 7.4,50% Glycerol.
    Derived fromTritirachium album limber
    Residue of deoxyribonucleaseusing λDNA as substrate, digest at 37 ℃ for more than 6 hours, no deoxyribonuclease activity was detected
    Residue of ribonucleasedigestion at 25℃for more than 16 hours, no ribonuclease activity was detected.

    Proteinase K Application

    Proteinase K is stable in urea and SDS, and also has the ability to degrade natural proteins, so it is widely used, mainly used in gene diagnostic kits, genomic DNA extraction kits, RNA extraction kits to remove DNA and RNA preparation nucleic acids Enzymes can also be used to extract non-protein components in tissues to degrade protein-containing impurities. For example, the preparation of DNA vaccines and heparin.

    Proteinase K Packaging 

    10g; 20g; 50g; 100g/bottle;

    Proteinase K Storage

    The dry powder state can be stored at low temperature under the condition of 0~4℃. After dissolving, dispense into appropriate volume and store at -15℃-25℃. Transport can be carried out at room temperature.
    Under the correct storage conditions, the dry powder state is valid for up to two years. The validity period of the liquid state at -15~25℃ is one year. After opening the package, please use it in time to prevent moisture absorption. After the solid enzyme is dissolved, it is recommended to filter and sterilize, divide it into an appropriate volume, and store at -15~25℃.